Composite

Part:BBa_K4644013:Design

Designed by: Han Cao   Group: iGEM23_BUCT   (2023-10-07)


pCS-lac-racE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 56
    Illegal NheI site found at 920
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 56
    Illegal BamHI site found at 902
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 56
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This gene fragment was then homologously recombined with the pcs27 plasmid backbone to obtain pcs-lac-racE.


Source

Bacillus amyloliquefaciens

References

Li, D.; Hou, L.; Gao, Y.; Tian,Z.; Fan, B.; Wang, F.; Li, S. Recent Advances in Microbial Synthesis of Poly-γ-Glutamic Acid: A Review. Foods 2022, 11, 739.